FLIM->TIMP

Fluorescence Lifetime Imaging Microscopy (FLIM) datasets collected using time-correlated single photon counting microscopes manufactured by the Becker & Hickl company are stored in a proprietary binary format with extension .sdt. In order to analyze such data with TIMP, pixels for analysis must be selected and the data must be converted to the ASCII format described here. The analysis of FLIM data with TIMP was described in the paper Fluorescence Lifetime Imaging Microscopy (FLIM) Data Analysis with TIMP.

In order to interactively view the contents of a .sdt file and select pixels from such a file that are of interest for analysis with TIMP, Sergey Laptenok developed the program below, which is an executable for MS Windows.